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Freeze-thaw cycles and cold methanol/water were used to extract the intracellular metabolites from the quenched cell pellets according to protocols described by Smart et al
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There's no evidence of receptor downregulation like with many hormones, but cycling is standard practice for research peptides and helps maintain sensitivity
[65] Dopamine contributes to the action selection process in at least two important ways
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CHS: Chalcone synthase
